dEPIMAL Project aims to study the relationship between antigenic stimulus upon Plasmodium infection and gene regulation of immune cells during pre-immunisation. Our goal is understanding how certain individuals from endemic regions acquire an effective natural immunity after years of repeated exposure to parasite infection. Differences in age and symptoms across individuals during this immune-protective process indicates that the epigenetic state of immune cells have a key role in the development of natural immunity.

Integrative Multi-Omics’ methods

    We use computational methods to integrate simultaneous gene expression and chromatin accessibility data from single-cell and bulk assays. Our approach consists of deploying genome-wide analyses to identify cell-specific regulatory signatures. In this project, we used data to study cell differentiation processes during immune response to malaria infection from murine models and humans.

Immunoproteomics of Pf-specific antigens

    We develop immunoproteomic methods to characterise antigen repertoires from individual serums with differential protective response to malaria. The identification of P. falciparum IgG and IgM antigenic variability in individuals by immunoproteomic-wide analysis will improve association studies of regulatory landscapes of immune cells.

Selected publications

  • 1. Reyes-Palomares A. et al. Remodeling of active endothelial enhancers is associated with aberrant gene-regulatory networks in pulmonary arterial hypertension. Nature Communications, 2020, vol. 11:1673.
  • 2. Azcarate et al. Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria. Sci Rep, 2020, vol. 10:9398.
  • 3. Berest et al. Quantification of differential transcription factor activity and multiomics-based classification into activators and repressors: diffTF. Cell Reports, 2019, vol. 29.
  • 4. Garg, S., Reyes-Palomares A. et al. Hepatic leukemia factor is a novel leukemic stem cell regulator in DNMT3A, NPM1, and FLT3-ITD triple-mutated AML. Blood, The Journal of the American Society of Hematology, 2019, vol. 134.
  • 5. de Mendoza et al. Screening for retroviruses and hepatitis viruses using dried blood spots reveals a high prevalence of occult hepatitis B in Ghana. Therapeutic advances in infectious disease, 2019, vol. 6.
  • 6. Azcarate et al. Iron supplementation in mouse expands cellular innate defences in spleen and defers lethal malaria infection. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 2017, vol. 1863.
  • 7. Kamali et al. Experimental immunization based on Plasmodium antigens isolated by antibody affinity. Journal of immunology research, 2015, vol. 2015.